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il5  (R&D Systems)


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    R&D Systems il5
    Il5, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il5/product/R&D Systems
    Average 94 stars, based on 59 article reviews
    il5 - by Bioz Stars, 2026-04
    94/100 stars

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    Eosinophil MYDGF. A, immunoblots of lysate from 2 × 105 unactivated (−) or <t>IL5-activated</t> (+) eosinophils per lane and 10 ng of recombinant, human MYDGF (rM) produced and purified from E. coli. Ponceau S stain is representative of total lysate protein; the most intense band centered at 13.4 kDa (*) corresponded to a band found in all immunoblot lanes regardless of whether goat anti-MYDGF, rabbit anti-MYDGF, or anti-P4HB primary antibodies were used. Mature MYDGF and P4HB were both detected in close proximity to their theoretical molecular masses of 15.8 and 55.3 kDa, respectively. All immunoblot images were taken with a 3-min exposure and the same image settings. B, human eosinophils, unactivated or activated with IL5, triple-stained with DAPI (blue), goat anti-human MYDGF or control IgGs (green), and rabbit anti-human MYDGF or control IgGs (pseudocolored red). Cells were imaged by confocal fluorescence and transmission microscopy. Images are of single 0.3-μm slices. Arrowheads, patch between nuclear lobes; arrows, periphery of nucleus; scale bar, 10 μm. Immunostaining and immunoblots are representative of experiments conducted on three or more separate occasions.
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    Eosinophil MYDGF. A, immunoblots of lysate from 2 × 105 unactivated (−) or <t>IL5-activated</t> (+) eosinophils per lane and 10 ng of recombinant, human MYDGF (rM) produced and purified from E. coli. Ponceau S stain is representative of total lysate protein; the most intense band centered at 13.4 kDa (*) corresponded to a band found in all immunoblot lanes regardless of whether goat anti-MYDGF, rabbit anti-MYDGF, or anti-P4HB primary antibodies were used. Mature MYDGF and P4HB were both detected in close proximity to their theoretical molecular masses of 15.8 and 55.3 kDa, respectively. All immunoblot images were taken with a 3-min exposure and the same image settings. B, human eosinophils, unactivated or activated with IL5, triple-stained with DAPI (blue), goat anti-human MYDGF or control IgGs (green), and rabbit anti-human MYDGF or control IgGs (pseudocolored red). Cells were imaged by confocal fluorescence and transmission microscopy. Images are of single 0.3-μm slices. Arrowheads, patch between nuclear lobes; arrows, periphery of nucleus; scale bar, 10 μm. Immunostaining and immunoblots are representative of experiments conducted on three or more separate occasions.
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    Eosinophil MYDGF. A, immunoblots of lysate from 2 × 105 unactivated (−) or <t>IL5-activated</t> (+) eosinophils per lane and 10 ng of recombinant, human MYDGF (rM) produced and purified from E. coli. Ponceau S stain is representative of total lysate protein; the most intense band centered at 13.4 kDa (*) corresponded to a band found in all immunoblot lanes regardless of whether goat anti-MYDGF, rabbit anti-MYDGF, or anti-P4HB primary antibodies were used. Mature MYDGF and P4HB were both detected in close proximity to their theoretical molecular masses of 15.8 and 55.3 kDa, respectively. All immunoblot images were taken with a 3-min exposure and the same image settings. B, human eosinophils, unactivated or activated with IL5, triple-stained with DAPI (blue), goat anti-human MYDGF or control IgGs (green), and rabbit anti-human MYDGF or control IgGs (pseudocolored red). Cells were imaged by confocal fluorescence and transmission microscopy. Images are of single 0.3-μm slices. Arrowheads, patch between nuclear lobes; arrows, periphery of nucleus; scale bar, 10 μm. Immunostaining and immunoblots are representative of experiments conducted on three or more separate occasions.
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    Image Search Results


    Eosinophil MYDGF. A, immunoblots of lysate from 2 × 105 unactivated (−) or IL5-activated (+) eosinophils per lane and 10 ng of recombinant, human MYDGF (rM) produced and purified from E. coli. Ponceau S stain is representative of total lysate protein; the most intense band centered at 13.4 kDa (*) corresponded to a band found in all immunoblot lanes regardless of whether goat anti-MYDGF, rabbit anti-MYDGF, or anti-P4HB primary antibodies were used. Mature MYDGF and P4HB were both detected in close proximity to their theoretical molecular masses of 15.8 and 55.3 kDa, respectively. All immunoblot images were taken with a 3-min exposure and the same image settings. B, human eosinophils, unactivated or activated with IL5, triple-stained with DAPI (blue), goat anti-human MYDGF or control IgGs (green), and rabbit anti-human MYDGF or control IgGs (pseudocolored red). Cells were imaged by confocal fluorescence and transmission microscopy. Images are of single 0.3-μm slices. Arrowheads, patch between nuclear lobes; arrows, periphery of nucleus; scale bar, 10 μm. Immunostaining and immunoblots are representative of experiments conducted on three or more separate occasions.

    Journal: The Journal of Biological Chemistry

    Article Title: Myeloid-derived growth factor is a resident endoplasmic reticulum protein

    doi: 10.1074/jbc.AC118.002052

    Figure Lengend Snippet: Eosinophil MYDGF. A, immunoblots of lysate from 2 × 105 unactivated (−) or IL5-activated (+) eosinophils per lane and 10 ng of recombinant, human MYDGF (rM) produced and purified from E. coli. Ponceau S stain is representative of total lysate protein; the most intense band centered at 13.4 kDa (*) corresponded to a band found in all immunoblot lanes regardless of whether goat anti-MYDGF, rabbit anti-MYDGF, or anti-P4HB primary antibodies were used. Mature MYDGF and P4HB were both detected in close proximity to their theoretical molecular masses of 15.8 and 55.3 kDa, respectively. All immunoblot images were taken with a 3-min exposure and the same image settings. B, human eosinophils, unactivated or activated with IL5, triple-stained with DAPI (blue), goat anti-human MYDGF or control IgGs (green), and rabbit anti-human MYDGF or control IgGs (pseudocolored red). Cells were imaged by confocal fluorescence and transmission microscopy. Images are of single 0.3-μm slices. Arrowheads, patch between nuclear lobes; arrows, periphery of nucleus; scale bar, 10 μm. Immunostaining and immunoblots are representative of experiments conducted on three or more separate occasions.

    Article Snippet: Goat anti-human MYDGF, sold as anti-human SF20 (AF1147), and IL5 (205-IL) were from R&D Systems (Bio-Techne Corp., Minneapolis, MN).

    Techniques: Western Blot, Recombinant, Produced, Purification, Staining, Control, Fluorescence, Transmission Assay, Microscopy, Immunostaining

    Analysis of MYDGF colocalization with organelle markers Colocalization was quantified as the area of overlapping signal from goat anti-MYDGF (Alexa Fluor 488–conjugated secondary antibody) and rabbit anti-MYDGF or rabbit antibodies to organelle markers (Alexa Fluor 647–conjugated secondary antibody) divided by the signal area from one antibody alone. Values are reported as averages from 9–16 0.3-μm slices from fields of 21–41 eosinophils that were overlaid to create the maximal intensity projections from which the cells shown in were chosen. Standard deviations represent variation among the slices.

    Journal: The Journal of Biological Chemistry

    Article Title: Myeloid-derived growth factor is a resident endoplasmic reticulum protein

    doi: 10.1074/jbc.AC118.002052

    Figure Lengend Snippet: Analysis of MYDGF colocalization with organelle markers Colocalization was quantified as the area of overlapping signal from goat anti-MYDGF (Alexa Fluor 488–conjugated secondary antibody) and rabbit anti-MYDGF or rabbit antibodies to organelle markers (Alexa Fluor 647–conjugated secondary antibody) divided by the signal area from one antibody alone. Values are reported as averages from 9–16 0.3-μm slices from fields of 21–41 eosinophils that were overlaid to create the maximal intensity projections from which the cells shown in were chosen. Standard deviations represent variation among the slices.

    Article Snippet: Goat anti-human MYDGF, sold as anti-human SF20 (AF1147), and IL5 (205-IL) were from R&D Systems (Bio-Techne Corp., Minneapolis, MN).

    Techniques:

    Colocalization of MYDGF and organelle markers. Staining of unactivated and IL5-activated eosinophils with DAPI (blue), goat anti-human MYDGF (green), and rabbit antibodies specific for organelle markers (pseudocolored red) is shown. Images are maximal intensity projections and representative of experiments conducted on three or more separate occasions. Scale bars, 10 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Myeloid-derived growth factor is a resident endoplasmic reticulum protein

    doi: 10.1074/jbc.AC118.002052

    Figure Lengend Snippet: Colocalization of MYDGF and organelle markers. Staining of unactivated and IL5-activated eosinophils with DAPI (blue), goat anti-human MYDGF (green), and rabbit antibodies specific for organelle markers (pseudocolored red) is shown. Images are maximal intensity projections and representative of experiments conducted on three or more separate occasions. Scale bars, 10 μm.

    Article Snippet: Goat anti-human MYDGF, sold as anti-human SF20 (AF1147), and IL5 (205-IL) were from R&D Systems (Bio-Techne Corp., Minneapolis, MN).

    Techniques: Staining